Reaktivität: Human, Maus, Ratte
FACS, IF (cc), IF (p)
For flow cytometry and innumihistochemistry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive HUVEC cells control
Nur für Forschungszwecke einsetzbar
PBS, containing 1 % bovine serum albumin and 0.02 % sodium azide 2* 3 1
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Informationen zur Lagerung
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
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The monoclonal antibody BV3 recognizes human alpha-V/beta-3 integrin present on human cells. Integrins are a superfamily of αβ heterodimeric cell-surface adhesion receptors found in many species. They are expressed on a variety of cells and mediate numerous physiological processes, including inflammation, migration, adhesion and proliferation. The β3 family consist of 2 members: αIIbβ3 and αvβ3, which mediate cell-cell and cell-ECM interactions and are important for cellular migration, regulation of gene expression, cell survival, adhesion and differentiation. All processes which are involved in tissue development, angiogenesis and thrombosis. Each subunit consist of an extracellular domain, a single transmembrane segment and a cytoplasmic tail. They connect to the actin cytoskeleton via adaptor proteins that bind theircytoplasmic tails. Cell matrix adhesions also act as signaling units by their capacity to organize the actin cytoskeleton and to accumulate various signaling intermediates. Integrin αvβ3 was originally identified as the vitronectin receptor. Nevertheless, other ligands include fibrinogen, fibronectin, laminin, thrombospondin, Von Willebrand factor, tenascin, osteopontin and several forms of collagen. The interactions of integrin αvβ3 to those ligands is mediated by the RGD (Arg-Gly-Asp) sequence motif present in these proteins. Deregulation of β3 integrins is involved in e.g. autoimmune diseases, cardiovascular disorders, transplant rejection and tumorigenesis. In contribution to the latter, integrin αvβ3 contribute by supporting growth of small (tumor) blood vessels thereby potentiating the metastatic potential. Overexpression of integrin αvβ3 has been demonstrated in various tumors and activated endothelium. Aliases Vitronectin receptor, integrin αvβ3, cd51/cd61