ERK2 Antikörper (C-Term)

Produktnummer ABIN218336

Der Kaninchen Polyklonal anti-ERK2 Antikörper wird verwendet zum Nachweis von ERK2 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC, IP und IHC (p).

Kurzübersicht für ERK2 Antikörper (C-Term) (ABIN218336)

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ERK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ERK2 Antikörper

Bindungsspezifität: C-Term

Spezifität: Peptide mapping to the carboxy terminus of rat ERK2

Aufreinigung: Affinity purified

Immunogen: Synthetic peptide from rat MAPK1 / ERK.

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (1:50), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:50.

Kommentare:

Target Species of Antibody: Rat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Phosphate-buffered solution,  pH 7.2, 0.09 % sodium azide, 50 % glycerol, 0.1 % gelatin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu ERK2

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Andere Bezeichnung: MAPK1 / ERK2 / p42

Hintergrund: Name/Gene ID: MAPK1
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK1, ERK, ERK-2, ERK2, MAP kinase 1, MAPK 2, MAPK2, MAP kinase isoform p42, MAPK 1, p41mapk, PRKM2, MAP kinase 2, p41, p42-MAPK, PRKM1, ERT1, p38, p40, p42MAPK, Protein tyrosine kinase ERK2

Gen-ID: 5594

UniProt: P28482

Pathways: MAPK Signalweg, RTK Signalweg, Apoptose, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagie, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteine