CD58 Antikörper (PE)

Produktnummer ABIN2144939

Produktdetails anti-CD58 Antikörper (PE)

Target: CD58

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD58 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Produktmerkmale: 4ATS2/9.1 reacts with 55-70 kDa CD58, lymphocyte function-associated antigen (LFA-3). It is expressed in hematipoietic and non-hematopoietic tissue including leukocytes, erythrocytes, endothelial cells, epithelial cells and fibroblasts.CD58 mediates adhesion between killer and target cells, antigen presenting cells and T cells, thymocytes and thymic epithelial cells. It also provides co-stimulatory signal in immune response.

Klon: 4ATS2-9-1-1-1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdurchführung:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu CD58

Target: CD58

Andere Bezeichnung: CD58 (CD58 Produkte)

Synonyme: LFA-3 antikoerper, LFA3 antikoerper, ag3 antikoerper, CD58 molecule antikoerper, CD58 antikoerper