CD56 Antikörper (PE)

Produktnummer ABIN2144931

Produktdetails anti-CD56 Antikörper (PE)

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD56 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS)

Produktmerkmale: 4AMEM188 reacts with CD56, a 175-220 kDa Neural Cell Adhesion Molecule (NCAM), expressed on 10-25 % of peripheral blood lymphocytes, including all CD16+ NK cells and approximately 5 % of CD3+ lymphocytes, referred to as NKT cells. It also is present at brain and neuromuscular junctions, certain LGL leukemias, small cell lung carcinomas, neuronally derived tumors, myeloma and myeloid leukemias. CD56 (NCAM) is involved in neuronal homotypic cell adhesion which is implicated in neural development, and in cell differentiation during embryogenesis.

Klon: 4A123C3

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdurchführung:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu CD56

Target: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Andere Bezeichnung: CD56 (NCAM1 Produkte)

Synonyme: CD56 antikoerper, MSK39 antikoerper, NCAM antikoerper, fj56g09 antikoerper, ncam antikoerper, ncam1 antikoerper, wu:fj56g09 antikoerper, NCAM1 antikoerper, n-cam antikoerper, E-NCAM antikoerper, NCAM-1 antikoerper, Ncam antikoerper, Cd56 antikoerper, N-CAM antikoerper, N-CAM-1 antikoerper, NCAM-C antikoerper, NCAMC antikoerper, N-CAM-1-B antikoerper, NCAM-1-B antikoerper, ncam1-a antikoerper, ncam1-b antikoerper, neural cell adhesion molecule 1 antikoerper, neural cell adhesion molecule 1a antikoerper, neural cell adhesion molecule 1 S homeolog antikoerper, neural cell adhesion molecule 1 L homeolog antikoerper, NCAM1 antikoerper, ncam1a antikoerper, ncam1 antikoerper, LOC100085373 antikoerper, ncam1.S antikoerper, Ncam1 antikoerper, ncam1.L antikoerper