CD45RO Antikörper (PE-Cy5)

Produktnummer ABIN2144389

Produktdetails anti-CD45RO Antikörper (PE-Cy5)

Target: CD45RO

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD45RO Antikörper ist konjugiert mit PE-Cy5

Applikation: Flow Cytometry (FACS)

Produktmerkmale: 4AUCHL1 reacts with an isoform of CD45, a 180 kDa molecule clustered as CD45RO.

Klon: 4AUCAL-1

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdurchführung:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Details zu CD45RO

Target: CD45RO

Andere Bezeichnung: CD45RO (CD45RO Produkte)

Hintergrund: CD45RO is expressed on memory/activated T cells and cortical thymocytes, in comparison with CD45RA, which is expressed on naive/resting T cells and on medullart thymocytes. CD45RO and CD45RA molecules are important to discriminate naive and memory T cells in the study of the immune system.