SERPINB9 Antikörper

Produktnummer ABIN206647

Produktdetails anti-SERPINB9 Antikörper

Target: SERPINB9 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 9 (SERPINB9))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SERPINB9 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Spezifität: Specific for human PI-9 (proteinase inhibitor 9), also known as SerpinB9, a 42kD intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells. Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virus-infected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves. Clone Detects a band of approximately 42kD under reducing conditions. Clone 7D8 is suitable for use in indirect Immunofluorescence.

Keine Kreuzreaktivität: Maus, Schwein

Aufreinigung: Protein G purified

Immunogen: Recombinant PI-9 produced in P. pastoris.

Type of Immunogen: Recombinant protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: Flo (1:10 - 1:100), IHC, IHC-P (1:50), WB

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Membrane permeabilization is required for this application. Immunohistochemistry: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer  pH 6.0 is recommended for this purpose. Histology Positive Control Tissue: Tonsil.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu SERPINB9

Target: SERPINB9 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 9 (SERPINB9))

Andere Bezeichnung: SERPINB9 / PI9 (SERPINB9 Produkte)

Synonyme: SERPINB9 antikoerper, BB283241 antikoerper, CAP-3 antikoerper, CAP3 antikoerper, PI-9 antikoerper, PI9 antikoerper, Spi6 antikoerper, ovalbumin antikoerper, serpin family B member 9 antikoerper, serpin peptidase inhibitor, clade B (ovalbumin), member 9 antikoerper, serine (or cysteine) peptidase inhibitor, clade B, member 9 antikoerper, Serpinb9 antikoerper, SERPINB9 antikoerper

Hintergrund: Name/Gene ID: SERPINB9

Synonyms: SERPINB9, CAP-3, CAP3, Cytoplasmic antiproteinase 3, Peptidase inhibitor 9, Serpin B9, PI-9, PI9

Gen-ID: 5272