Kininogen LC (AA 1-406) Antikörper

Produktnummer ABIN1885922

Produktdetails

Target: Kininogen LC

Bindungsspezifität: AA 1-406

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 406 of Human KNG1

Anwendungsinformationen

Applikationshinweise: Suggested dilutions:
Western blotting: 1.500-1.3000
Immunohistochemistry: 1.50-1.500
Immunofluorescence: 1.100-1.200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 0.1 M Tris-buffered saline with 20 % Glycerol (pH 7.0).0.01 % Thimerosal was added as a preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: Biohazard Informations: This product contains thimerosal which is hazardous.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Antigendetails

Target: Kininogen LC

Hintergrund: High molecular weight kininogen (HMWK) plays an important role in assembly of the plasma kallikrein (see MIM 147910)-kinin system.The KNG1 gene generates both HMWK and low molecular weight kininogen (LMWK) through alternative splicing.Both HMWK and LMWK contain an identical heavy chain consisting of protein domains 1, 2, and 3.However, HMWK contains a 56-kD light chain that consists of domains 5 and 6H, whereas LMWK contains a unique 4-kD light chain that consists of domain 5L.In both proteins, the heavy and light chains are linked by domain 4, which contains the bradykinin (BK) nonapeptide.BK, which is released by plasma kallikrein, is a potent inflammatory mediator that causes vasodilation and enhanced capillary permeability, induces pain, and stimulates production of nitric oxide and prostacyclin (see MIM 601699) from endothelial cells.During vascular damage, BK stimulates smooth muscle proliferation and intimal hypertrophy.Release of BK from HMWK generates a 2-chain HMWK, termed HMWKa, containing the heavy and light chains joined by a disulfide bond (Merkulov et al., 2008 [PubMed 18000168]).[supplied by OMIM]

Molekulargewicht: 48 kDa

Gen-ID: 3827

NCBI Accession: NP_000884, NM_000893