beta-Adrenergic Receptor Kinase (AA 398-640) Antikörper

Produktnummer ABIN1857927

Produktdetails

Target: beta-Adrenergic Receptor Kinase

Bindungsspezifität: AA 398-640

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)

Verwendungszweck: Polyclonal Antibody to Beta Adrenergic Receptor Kinase (BARK)

Spezifität: The antibody is a rabbit polyclonal antibody raised against BARK. It has been selected for its ability to recognize BARK in immunohistochemical staining and western blotting.

Kreuzreaktivität: Maus

Aufreinigung: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Beta Adrenergic Receptor Kinase (BARK) corresdonding to Asp398~Leu640 (Accession # P25098) with N-terminal His and GST Tag

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 24 months

Antigendetails

Target: beta-Adrenergic Receptor Kinase

Andere Bezeichnung: Beta Adrenergic Receptor Kinase

Synonyme: BARK1 antikoerper, BETA-ARK1 antikoerper, GRK2 antikoerper, G protein-coupled receptor kinase 2 antikoerper, GRK2 antikoerper

Hintergrund: ADRbK1, B-ARK1, bARK1, Beta-ARK1, GRK2, G-protein coupled receptor kinase 2, Adrenergic Receptor Beta Kinase 1