GalphaO 1/2 Antikörper

Produktnummer ABIN1742532

Produktdetails

Target: GalphaO 1/2

Reaktivität: Maus, Ratte, Zebrafisch (Danio rerio)

Wirt: Maus

Klonalität: Monoklonal

Applikation: Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)

Spezifität: Recognizes GalphaO 1 and GalphaO 2

Aufreinigung: purified IgG

Immunogen: Recombinant full length rodent GalphaO 2.

Klon: 101-1

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: WB: 1 : 1000 (AP staining)
ICC: 1 : 500 up to 1 : 1000
IHC: not tested yet

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handhabung: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Lagerung: -20 °C

Informationen zur Lagerung: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Referenzen

Baron, Blex, Rohrbeck, Rachakonda, Birnbaumer, Ahnert-Hilger, Brunk: "The α-subunit of the trimeric GTPase Go2 regulates axonal growth." in: Journal of neurochemistry, Vol. 124, Issue 6, pp. 782-94, (2013) (PubMed).

Winter, Brunk, Walther, Höltje, Jiang, Peter, Takamori, Jahn, Birnbaumer, Ahnert-Hilger: "Galphao2 regulates vesicular glutamate transporter activity by changing its chloride dependence." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 25, Issue 18, pp. 4672-80, (2005) (PubMed).

Antigendetails

Target: GalphaO 1/2