GABRA1 Antikörper (AA 28-43)
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- Target Alle GABRA1 Antikörper anzeigen
- GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))
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Bindungsspezifität
- AA 28-43
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Reaktivität
- Ratte, Maus
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Wirt
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Meerschweinchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser GABRA1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
- Spezifität
- Specific for GABA-A receptor alpha1.
- Aufreinigung
- antiserum
- Immunogen
- Synthetic peptide (aa 28-43 of rat GABA-A receptor alpha1 precursor protein) coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue.
- Top Product
- Discover our top product GABRA1 Primärantikörper
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- Applikationshinweise
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WB: 1 : 1000 up to 1 : 2000 (AP staining)
IP: not tested yet
ICC: 1 : 200 up to 1 : 1000
IHC: 1 : 500 - Kommentare
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WB: This protein aggregates after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ICC: This antibody is PFA fixation sensitive, use only mild fixation (2% PFA). Best results are obtained by application on living cells. After washing cells with bound antibodies, they can be fixed and visualized with secondary reagents. IHC: This antibody requires antigen retrieval with pepsin according to: Lorincz A & Nusser Z (2008).
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- PBS
- Handhabung
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Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable. - Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.
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- Target
- GABRA1 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 1 (GABRA1))
- Andere Bezeichnung
- GABA-A Receptor Alpha1 (GABRA1 Produkte)
- Synonyme
- ECA4 antikoerper, EJM antikoerper, EJM5 antikoerper, zgc:153566 antikoerper, Gabra-1 antikoerper, gamma-aminobutyric acid type A receptor alpha1 subunit antikoerper, gamma-aminobutyric acid (GABA) A receptor, alpha 1 antikoerper, gamma-aminobutyric acid (GABA) A receptor, subunit alpha 1 antikoerper, gamma-aminobutyric acid receptor subunit alpha-1 antikoerper, GABRA1 antikoerper, Gabra1 antikoerper, gabra1 antikoerper, LOC100653509 antikoerper
- Hintergrund
- Synonyms: GABA(A) R alpha1
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