Abeta 38/40/42 (AA 1-16), (AA 4-16) Antikörper

Produktnummer ABIN1742437

Produktdetails

Target: Abeta 38/40/42

Bindungsspezifität: AA 1-16, AA 4-16

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), ELISA

Spezifität: Specific for Abeta 38, 40, 42.

Aufreinigung: purified IgG. Azide was added before lyophilization.

Immunogen: Synthetic peptide (aa 1-16) of abeta peptides 38, 40, 42 coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue. Epitope aa 4-16

Klon: NT78

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: WB: 1 : 500 up to 1 : 1000
IP: not tested yet
ICC: not tested yet
IHC-P: 1 : 200 up to 1 : 500

Kommentare:

ELISA: Suitable as capture antibody for sandwich-ELISA as detector antibody (protocol for sandwich-ELISA).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Lagerung: -20 °C

Informationen zur Lagerung: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Referenzen

Jawhar, Wirths, Schilling, Graubner, Demuth, Bayer et al.: "Overexpression of glutaminyl cyclase, the enzyme responsible for pyroglutamate A{beta} formation, induces behavioral deficits, and glutaminyl cyclase knock-out rescues the behavioral phenotype in ..." in: The Journal of biological chemistry, Vol. 286, Issue 6, pp. 4454-60, (2011) (PubMed).

Wirths, Erck, Martens, Harmeier, Geumann, Jawhar, Kumar, Multhaup, Walter, Ingelsson, Degerman-Gunnarsson, Kalimo, Huitinga, Lannfelt, Bayer: "Identification of low molecular weight pyroglutamate A{beta} oligomers in Alzheimer disease: a novel tool for therapy and diagnosis." in: The Journal of biological chemistry, Vol. 285, Issue 53, pp. 41517-24, (2010) (PubMed).

Antigendetails

Target: Abeta 38/40/42