Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.
Immunogen
Synthetic peptide of mouse GluA 3 (aa 860-871) coupled to KHL via an N-terminal cystein.
Applikationshinweise
WB: 1 : 1000 (AP staining) ICC: not tested yet IHC: not tested yet
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.
Buffer
PBS
Handhabung
Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.
Lagerung
-20 °C
Informationen zur Lagerung
Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!
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Schmeisser, Ey, Wegener, Bockmann, Stempel, Kuebler, Janssen, Udvardi, Shiban, Spilker, Balschun, Skryabin, Dieck, Smalla, Montag, Leblond, Faure, Torquet, Le Sourd, Toro, Grabrucker, Shoichet et al.: "Autistic-like behaviours and hyperactivity in mice lacking ProSAP1/Shank2. ..." in: Nature, Vol. 486, Issue 7402, pp. 256-60, (2012) (PubMed).