CACNA1B Antikörper (AA 1921-2222)

Produktnummer ABIN1742328

Produktdetails anti-CACNA1B Antikörper

Target: CACNA1B (Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B))

Bindungsspezifität: AA 1921-2222

Reaktivität: Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CACNA1B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Spezifität: Specific for Ca2+ channel alpha-1E.

Aufreinigung: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Recombinant protein (aa 1921-2222 of rat Ca2+-channel alpha-1E subunit).

Anwendungsinformationen

Applikationshinweise: WB: 1 : 500 up to 1 : 1000
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500

Kommentare:

WB: Due to its large size, this antibody requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting. Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed or NuPage TRIS-acetate gels from Invitrogen. WB: This protein tends to aggregate after boiling, making it necessary to run SDS-PAGE with non-boiled samples. ECL detection is recommended: IHC: This antibody requires a mild fixation protocol (1 % PFA in sodium acetate buffer, pH 6).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handhabung: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Lagerung: -20 °C

Informationen zur Lagerung: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

Details zu CACNA1B

Target: CACNA1B (Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B))

Andere Bezeichnung: Ca2+ Channel (CACNA1B Produkte)

Synonyme: BIII antikoerper, CACNL1A5 antikoerper, CACNN antikoerper, Cav2.2 antikoerper, AW050276 antikoerper, AW060892 antikoerper, AW822256 antikoerper, Cchn1a antikoerper, alpha(1B) antikoerper, calcium voltage-gated channel subunit alpha1 B antikoerper, calcium channel, voltage-dependent, N type, alpha 1B subunit antikoerper, CACNA1B antikoerper, Cacna1b antikoerper

Hintergrund: Synonyms: Cav2.3