Proton ATPase (AA 826-838) Antikörper

Produktnummer ABIN1742216

Produktdetails

Target: Proton ATPase

Bindungsspezifität: AA 826-838

Reaktivität: Hamster, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Spezifität: Specific for the alpha1 116kDa subunit.

Aufreinigung: antiserum

Immunogen: Synthetic peptide FSFEHIREGKFDE (826-838 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue.

Anwendungsinformationen

Applikationshinweise: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 100
IHC-P: 1 : 100

Kommentare:

WB: The proton pump aggregates after boiling, making it necessary to run SDS-PAGE only with non-boiled samples. ICC: Methanol fixation is recommended.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS

Handhabung: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

Referenzen

Gouzy, Larrouy-Maumus, Bottai, Levillain, Dumas, Wallach, Caire-Brandli, de Chastellier, Wu, Poincloux, Brosch, Guerquin-Kern, Schnappinger, Sório de Carvalho, Poquet, Neyrolles: "Mycobacterium tuberculosis exploits asparagine to assimilate nitrogen and resist acid stress during infection." in: PLoS pathogens, Vol. 10, Issue 2, pp. e1003928, (2014) (PubMed).

Yao, de la Iglesia, Bajjalieh: "Loss of the SV2-like protein SVOP produces no apparent deficits in laboratory mice." in: PLoS ONE, Vol. 8, Issue 7, pp. e68215, (2013) (PubMed).

Antigendetails

Target: Proton ATPase