Human Papilloma Virus 16 E6 (HPV-16 E6) (AA 85-158) Antikörper (Alexa Fluor 350)

Produktnummer ABIN1692573

Produktdetails

Target: Human Papilloma Virus 16 E6 (HPV-16 E6)

Bindungsspezifität: AA 85-158

Reaktivität: Human Papillomavirus 16 (HPV-16), Virus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Alexa Fluor 350

Applikation: Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Kreuzreaktivität: Virus

Kreuzreaktivität (Details): HPV16

Aufreinigung: Purified by Protein G.

Immunogen: KLH conjugated synthetic peptide derived from HPV16 E6 protein

Klon: 2E3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Human Papilloma Virus 16 E6 (HPV-16 E6)

Andere Bezeichnung: HPV16 E6 protein

Substanzklasse: Viral Protein

Hintergrund:

Synonyms: Protein E6, E6

Background: Plays a major role in the induction and maintenance of cellular transformation. Acts mainly as an oncoprotein by stimulating the destruction of many host cell key regulatory proteins. E6 associates with host E6-AP ubiquitin-protein ligase, and inactivates tumor suppressors TP53 and TP73 by targeting them to the 26S proteasome for degradation. In turn, DNA damage and chromosomal instabilities increase and lead to cell proliferation and cancer development. The complex E6/E6P targets several other substrates to degradation via the proteasome including host NFX1-91, a repressor of human telomerase reverse transcriptase (hTERT). The resulting increased expression of hTERT prevents the shortening of telomere length leading to cell immortalization. Other cellular targets including Bak, Fas-associated death domain-containing protein (FADD) and procaspase 8, are degraded by E6/E6AP causing inhibition of apoptosis. E6 also inhibits immune response by interacting with host IRF3 and TYK2. These interactions prevent IRF3 transcriptional activities and inhibit TYK2-mediated JAK-STAT activation by interferon alpha resulting in inhibition of the interferon signaling pathway.

Gen-ID: 1489078

UniProt: P03126