ARHGAP22 Antikörper (phosphorylated)

Produktnummer ABIN1607879

Produktdetails anti-ARHGAP22 Antikörper

Target: ARHGAP22 (rho GTPase Activating Protein 22 (ARHGAP22))

Bindungsspezifität: phosphorylated

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ARHGAP22 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Fluorescence Microscopy (FM)

Kreuzreaktivität (Details): Cross reactivity with ARHGAP22 pS397 from other sources has not been determined.

Reinheit: Anti-ARHGAP22 pS397 was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody is specific for phosphorylated ARHGAP22 at Serine 397. It also recognizes the S22-> A mutation but not the S397-> A mutation. A BLAST analysis was used to suggest cross-reactivity with Mouse, Rat and Human based on 100 % sequence homology.

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: ARHGAP22 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the region surrounding mouse pS397 region of ARHGAP22.
Immunogen Type: Peptide

Anwendungsinformationen

Applikationshinweise: Anti-ARHGAP22 pS397 antibody is useful for ELISA, Western Blot and Immunostaining. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~77.8 kDa corresponding to the appropriate cell lysate or extract.
ELISA Dilution: 1:20.000 - 1:60.000
Immunohistochemistry Dilution: 1:100-1:500
IF Microscopy Dilution: 1:100-1:500
Western Blot Dilution: 1 µg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.07 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 50 % (v/v) Glycerol

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: Expiration date is six (6) months from date of opening.

Details zu ARHGAP22

Target: ARHGAP22 (rho GTPase Activating Protein 22 (ARHGAP22))

Andere Bezeichnung: ARHGAP22 (ARHGAP22 Produkte)

Synonyme: si:ch211-223a10.2 antikoerper, RhoGAP2 antikoerper, RhoGap22 antikoerper, B230341L19Rik antikoerper, RHOGAP2 antikoerper, p68RacGAP antikoerper, Rho GTPase activating protein 22 antikoerper, rho GTPase activating protein 22 antikoerper, ARHGAP22 antikoerper, arhgap22 antikoerper, CC1G_06014 antikoerper, Arhgap22 antikoerper

Hintergrund: ARHGAP22 is a Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. It acts as a GTPase activator for RAC1 by converting it to an inactive GDP-bound state and also inhibits RAC1-dependent lamellipodia formation. It may also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter. Anti-ARHGAP22 [p Ser397] antibody is ideal for researchers interested in Diabetes Research, Lipid and Metabolism research. Synonyms: Rho-type GTPase-activating protein 22, RHOGAP2, RhoGAP2, Rho GTPase activating protein 22, rho GTPase-activating protein 22

Gen-ID: 239027

NCBI Accession: NP_722495

UniProt: Q8BL80