Maus anti-Human IgG (Fc Region) Antikörper

Produktnummer ABIN1607856

Produktdetails

Target: IgG

Bindungsspezifität: Fc Region

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Applikation: ELISA, Western Blotting (WB)

Spezifität: IgG F(c)

Kreuzreaktivität: Human

Aufreinigung: Anti-Human IgG F(c) Antibody was prepared from concentrated roller bottle supernatant by Protein A chromatography sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgG, Human Serum and Human F(c).  No reaction was observed against Human F(ab).

Immunogen:

Immunogen: Anti-Human IgG F(c) monoclonal antibody was produced by repeated immunization with Human IgG F(c) fragment in mice.

Immunogen Type: Native Protein

Klon: 3D8-H7-D4

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Application Note: Human IgG F(c) antibody is suitable for immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

ELISA Dilution: 1-:1000-1:10000

Western Blot Dilution: 1:1000 - 1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid cycles of freezing and thawing.
Do NOT add Sodium Azide!
Centrifuge product if not completely clear after standing at room temperature.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 4 °C as an undiluted liquid.

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund:

Synonyms: Human IgG F(c) Antibody, Human IgG Fc Antibody, Ms-a-Human IgG Fc, Human IgG F(c) Antibody in Mouse, Human Secondary Antibody

Background: Anti-Human IgG F(c) generated in mouse detects Human F(c). A proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(c) Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.