Fructosyl Amino Acid Oxidase Antikörper (Biotin)

Produktnummer ABIN1607845

Produktdetails anti-Fructosyl Amino Acid Oxidase Antikörper (Biotin)

Target: Fructosyl Amino Acid Oxidase (socD)

Reaktivität: Corynebacterium

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser Fructosyl Amino Acid Oxidase Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Kreuzreaktivität (Details): Cross reactivity against Fructosyl Amino Acid Oxidase from other sources is unknown.

Reinheit: Anti-Fructosyl-Amino Acid Oxidase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Fructosyl Amino Acid Oxidase [E.coli].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Fructosyl-Amino Acid Oxidase [from Corynebacterium sp. Expressed in E.coli]
Immunogen Type: Recombinant Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-Fructosyl-Amino Acid Oxidase Antibody has been assayed against 1.0 µg of Fructosyl Amino Acid Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:8.000 to 1:34.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu Fructosyl Amino Acid Oxidase

Target: Fructosyl Amino Acid Oxidase (socD)

Andere Bezeichnung: Fructosyl-Amino Acid Oxidase (socD Produkte)

Synonyme: fructosyl amino acid oxidase antikoerper, hypothetical protein antikoerper, socD antikoerper, CNB00220 antikoerper, AFUA_8G06440 antikoerper, AFUA_6G03440 antikoerper, PTRG_01708 antikoerper, ANI_1_1464184 antikoerper, AOR_1_60064 antikoerper, PGTG_09157 antikoerper

Hintergrund: Anti-Fructosyl-Amino Acid Oxidase antibody catalyzes the oxidative deglycation of fructosyl amino acids to produce the corresponding amino acid, glucosone, and hydrogen peroxide. Anti-Fructosyl-Amino Acid Oxidase antibodies are ideal for researchers interested in Metabolism and Signal Transduction research. Synonyms: Fructosyl-amino acid oxidase EMBL BAB91123.1 EC=1.5.3

UniProt: Q8RIU8