CPA Antikörper (Biotin)

Produktnummer ABIN1607785

Produktdetails anti-CPA Antikörper (Biotin)

Target: CPA (Carboxypeptidase A (CPA))

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CPA Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität (Details): Cross reactivity against Carboxypeptidase A from other sources may occur but have not been specifically determined.

Reinheit: Anti-Carboxypeptidase A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase A [Bovine Pancreas].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Carboxypeptidase A [Bovine Pancreas]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-Carboxypeptidase A has been assayed against 1.0 µg of Carboxypeptidase A in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:15.000 to 1:55.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu CPA

Target: CPA (Carboxypeptidase A (CPA))

Andere Bezeichnung: Carboxypeptidase A (CPA Produkte)

Hintergrund: Anti-Carboxypeptidase A antibody catalyzes the release of C-terminal amino acids with little or no action with –Asp, -Glu, -Arg, -Lys or –Pro. This antibody is ideal for investigators interested in Metabolism, Cancer, Signal Transduction, or Cell Biology research. Synonyms: Carboxypeptidase A1 precursor antibody, CPA1 antibody, Pancreatic carboxypeptidase A1 antibody, Procarboxypeptidase A1 pancreatic antibody

Gen-ID: 286762

UniProt: P00730