Lactate Dehydrogenase Antikörper (Biotin)

Produktnummer ABIN1607671

Produktdetails anti-Lactate Dehydrogenase Antikörper (Biotin)

Target: Lactate Dehydrogenase (LDH)

Reaktivität: Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Lactate Dehydrogenase Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Kreuzreaktivität (Details): Cross reactivity against Lactate Dehydrogenase from other sources may occur but have not been specifically determined.

Reinheit: Anti-lactate dehydrogenase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Lactate Dehydrogenase [full length protein isolated from rabbit muscle]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-lactate dehydrogenase antibody has been assayed against 1.0 µg of Lactate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Referenzen für anti-Lactate Dehydrogenase Antikörper (Biotin) (ABIN1607671)

Vincent, Cornea, Jong, Laferrière, Kumar, Mickeviciute, Fung, Bandegi, Ribeiro-da-Silva, OMalley, Coderre: "Intracellular mGluR5 plays a critical role in neuropathic pain." in: Nature communications, Vol. 7, pp. 10604, (2016) (PubMed).

Details zu Lactate Dehydrogenase

Target: Lactate Dehydrogenase (LDH)

Andere Bezeichnung: Lactate Dehydrogenase (LDH Produkte)

Synonyme: CG10160 antikoerper, Dmel\\CG10160 antikoerper, IMP-L3 antikoerper, IMPL3 antikoerper, Imp-L3 antikoerper, Impl3 antikoerper, LDH antikoerper, Ld antikoerper, Ldh antikoerper, dLdha antikoerper, dmLDH antikoerper, Ecdysone-inducible gene L3 antikoerper, lactate dehydrogenase antikoerper, ldh2 antikoerper, ImpL3 antikoerper, ldh antikoerper, ldh2 antikoerper, Ldh antikoerper, LDH-A antikoerper

Hintergrund: Anti-lactate dehydrogenase recognizes lactate dehydrogenase which catalyzes the interconversion of pyruvate to lactate and NADH to NAD+. This reaction occurs in the absence or lack of oxygen, making lactate dehydrogenase critical for anerobic respiration. There five known isozymes of LDH, each localized in different types of tissue. In medicine, LDH is often used as a marker of various diseases ranging from hemolysis to encephalitis. Anti-lactate dehydrogenase antibody is ideal for investigators interested in Metabolism, Cancer, and Signal Transduction. Synonyms: L-lactate dehydrogenase A chain LDH-A LDH muscle subunit LDH-M

Gen-ID: 100009107, 126050

UniProt: P13491