CA1 Antikörper (Biotin)

Produktnummer ABIN1607668

Produktdetails anti-CA1 Antikörper (Biotin)

Target: CA1 (Carbonic Anhydrase I (CA1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CA1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität (Details): Cross reactivity against Carbonic Anhydrase I from other sources may occur but has not been specifically determined.

Reinheit: Anti-CARBONIC ANHYDRASE I (GOAT) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Carbonic Anhydrase I [Human Erythrocytes].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Carbonic Anhydrase I [Human Erythrocytes]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-CARBONIC ANHYDRASE I (GOAT) Antibody has been assayed against 1.0 µg of Carbonic Anhydrase I in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product. Specific conditions should be optimized by researcher.
ELISA Dilution: 1:20.000 - 1:100.000
Western Blot Dilution: 1:2.000 - 1:10.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu CA1

Target: CA1 (Carbonic Anhydrase I (CA1))

Andere Bezeichnung: Carbonic Anhydrase I (CA1 Produkte)

Synonyme: CA-I antikoerper, CAB antikoerper, Car1 antikoerper, AW555628 antikoerper, Ca1 antikoerper, Car-1 antikoerper, BG:DS00941.1 antikoerper, CAH antikoerper, CG7820 antikoerper, Dmel\\CG7820 antikoerper, Gh7 antikoerper, ARABIDOPSIS THALIANA SALICYLIC ACID-BINDING PROTEIN 3 antikoerper, ATBCA1 antikoerper, ATSABP3 antikoerper, BETA CARBONIC ANHYDRASE 1 antikoerper, F4P13.5 antikoerper, F4P13_5 antikoerper, SABP3 antikoerper, SALICYLIC ACID-BINDING PROTEIN 3 antikoerper, carbonic anhydrase 1 antikoerper, CA1 antikoerper, LOC100135826 antikoerper, GB15888 antikoerper, carbonic anhydrase 1 antikoerper, Carbonic anhydrase 1 antikoerper, carbonic anhydrase I antikoerper, carbonic anhydrase antikoerper, CA1 antikoerper, Car1 antikoerper, CAH1 antikoerper, LOC100135826 antikoerper, LOC408827 antikoerper, Tsp_05114 antikoerper, LOC100153915 antikoerper, LOC100050192 antikoerper, Ca1 antikoerper

Hintergrund: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. Carbonic Anhydrase 1 (CA1) is closely related to Carbonic Anhydrase 2 (CA2) and Carbonic Anhydrase 3 (CA3), and it is a cytosolic protein that is found at the highest level in erythrocytes. Anti-Carbonic anhydrase I antibody is ideal for investigators involved in serum protein components. Synonyms: Car1 antibody, Carbonate dehydratase I antibody, Carbonic anhydrase 1 antibody, Carbonic anhydrase B antibody, Carbonic dehydratase antibody, ECK0125 antibody, yadF antibody

Gen-ID: 759

UniProt: P00915