MYH6 Antikörper

Produktnummer ABIN1607601

Produktdetails anti-MYH6 Antikörper

Target: MYH6 (Myosin Heavy Chain 6, Cardiac Muscle, alpha (MYH6))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MYH6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität (Details): Cross reactivity with myosin6 from other sources have not been determined.

Reinheit: Anti-Myosin-6 is directed against the human myosin-6 protein. The product was prepared from monospecific antiserum by delipidation and defibrination. A BLAST analysis was used to suggest reactivity with human only.

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Anti-Myosin-6 was prepared from whole rabbit serum produced by repeated immunizations with a truncated myosin-6 construct expressed in SF9 insect cells corresponding to human myosyn-6 protein.
Immunogen Type: Recombinant Protein

Anwendungsinformationen

Applikationshinweise: Myosin-6 antibody has been tested for use in ELISA and western blot. For western blots expect a band of approximately 150 kDa in size corresponding to truncated myosin-6 protein. Specific conditions for reactivity should be optimized by the end user.
ELISA Dilution: 1:10.000
Western Blot Dilution: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 25 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu MYH6

Target: MYH6 (Myosin Heavy Chain 6, Cardiac Muscle, alpha (MYH6))

Andere Bezeichnung: Myosin 6 (MYH6 Produkte)

Synonyme: Myhca antikoerper, ASD3 antikoerper, CMD1EE antikoerper, CMH14 antikoerper, MYHC antikoerper, MYHCA antikoerper, SSS3 antikoerper, alpha-MHC antikoerper, A830009F23Rik antikoerper, AA517445 antikoerper, Myhc-a antikoerper, amhc antikoerper, MYH6 antikoerper, P-MHC antikoerper, VMHC1 antikoerper, myosin antikoerper, myosin heavy chain 6 antikoerper, myosin, heavy polypeptide 6, cardiac muscle, alpha antikoerper, myosin, heavy chain 6, cardiac muscle, alpha antikoerper, myosin-6 antikoerper, myosin, heavy chain 15 antikoerper, Myh6 antikoerper, MYH6 antikoerper, LOC101120580 antikoerper, myh6 antikoerper, MYH15 antikoerper

Hintergrund: Myosin VI is a myosin superfamily member with unique and intriguing features that allow it to fill a still-expanding number of cell biological roles. This actin-based motor produces force that acts towards the minus end of actin filaments, which is the opposite direction to all other characterized myosins. In mammalian cells, myosin VI is localized to endocytic vesicles, membrane ruffles, the cytosol and the Golgi complex. Its motor function is essential for several physiological functions of the cell, including normal rates of endocytosis, maintenance of Golgi morphology and protein secretion. Myosin VI regulates epithelial cell migration and plays a role in the maintenance of adhesive cellular contacts within epithelial cell layers. It is highly expressed in ovarian cancers and prostate cancers and its expression level, which is upregulated by DNA damage in a p53-dependent manner, correlates with the potential of the tumor to disseminate. More recently, myosin VI has been found involved in EGFR endocytosis through a clathrin dependent mechanism. Synonyms: MyosinVI, Unconventional myosin 6

Gen-ID: 3799

NCBI Accession: NP_004512

UniProt: P33176