P35 (N-Term) Antikörper

Produktnummer ABIN1574113

Produktdetails

Target: P35

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB)

Spezifität: Rabbit Anti-p35 Polyclonal Antibody detects endogenous levels of mouse and rat p35. It is predicted to react with human p35 according to sequence homology.Positive Control: mouse brain, and rat brain.

Kreuzreaktivität (Details): Rabbit Anti-p35 Polyclonal Antibody detects endogenous levels of mouse and rat p35. It is predicted to react with human p35 according to sequence homology.Positive Control: mouse brain, and rat brain.

Aufreinigung: Immunoaffinity chromatography

Immunogen: KLH-coupled synthetic peptide from N-terminal of human p35 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 1-2 µg/mLOther Applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: P35

Synonyme: Anx1 antikoerper, p35 antikoerper, CDK5P35 antikoerper, CDK5R antikoerper, NCK5A antikoerper, p23 antikoerper, p25 antikoerper, p35nck5a antikoerper, 2010323F13Rik antikoerper, GFRP antikoerper, P35 antikoerper, annexin A1 antikoerper, cyclin dependent kinase 5 regulatory subunit 1 antikoerper, GTP cyclohydrolase I feedback regulator antikoerper, Anxa1 antikoerper, CDK5R1 antikoerper, Gchfr antikoerper

Substanzklasse: Viral Protein

Hintergrund: Cyclin-dependent kinase 5 (Cdk5) is a member of the Cdk family of proteins that are involved in the regulation of the mitotic cell cycle. p35 protein is neuronal-specific regulatory subunit of Cdk5 and activates Cdk5 via its direct interaction. p35/Cdk5 activity is essential for neuronal morphogenesis and survival. p35 is cleaved by the Ca2+ dependent Cys protease calpain, which results in the generation of a C-terminal truncation product named p25. Containing all elements necessary for the activation of Cdk5, p25 activates Cdk5. Generation of p25 in the nervous system has been implicated in patients with Alzheimer-disease. It is suggested that p25/Cdk5 contributes to neuronal cell death after neurotoxic insults.Rabbit Anti-p35 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from N-terminal of human p35 protein (Swiss Prot: Q15078).