Glu-Glu Tag Antikörper

Produktnummer ABIN1573897

Produktdetails anti-Glu-Glu Tag Antikörper

Target: Glu-Glu Tag

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Glu-Glu Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Sequenz: EYMPME

Spezifität: Rabbit Anti-Glu-Glu--tag Polyclonal Antibody recognizes either internal or C-terminal Glu-Glu tagged fusion proteins.

Aufreinigung: Immunoaffinity chromatography

Immunogen: Glu-Glu epitope tag peptide (EYMPME) conjugated - KLH

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, detection method sensitivity, temperature, incubations length, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are starting recommendations for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 1-2 µg/mL
Other applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Referenzen für anti-Glu-Glu Tag Antikörper (ABIN1573897)

Beach, Smith, Ramamoorthy, Meng et al.: "Chimeric porcine circoviruses (PCV) containing amino acid epitope tags in the C terminus of the capsid gene are infectious and elicit both anti-epitope tag antibodies and anti-PCV type 2 neutralizing ..." in: Journal of virology, Vol. 85, Issue 9, pp. 4591-5, (2011) (PubMed).

Details zu Glu-Glu Tag

Target: Glu-Glu Tag

Andere Bezeichnung: Glu-Glu-tag (Glu-Glu Tag Produkte)

Substanzklasse: Tag

Hintergrund: Well-characterized antibodies for epitope tags consisting of short sequences are widely used in the study of protein expression in various systems.The Glu-Glu-tag is an epitope tag consisting of a six-residue peptide, EYMPME, derived from the polyoma virus medium T antigen.Rabbit Anti-Glu-Glu-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic Glu-Glu-tag epitope peptide conjugated to KLH.