PKC alpha Antikörper (pThr638)

Produktnummer ABIN1531462

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PKC alpha in WB, IHC und ELISA. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 1 Publikation erwähnt.

Kurzübersicht für PKC alpha Antikörper (pThr638) (ABIN1531462)

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PKC alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-PKC alpha Antikörper

Bindungsspezifität: AA 606-655, pThr638

Spezifität:

PKC alpha (Phospho-Thr638) Antibody detects endogenous levels of PKC alpha only when phosphorylated at Thr638.

PhosphorylationH:T638 M:T638 R:T638

Aufreinigung: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Reinheit: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human PKC alpha around the phosphorylation site of Thr638.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

Kommentare:

Unigene-Number: Hs.531704, Hs.708867 (NCBI Gene Symbol: PRKCA)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable at -20°C for at least 1 year.

Haltbarkeit: 12 months

Referenzen für anti-PKC alpha Antikörper (ABIN1531462)

Huang, Cheng, Su, Lin: "Syndecan-4 Promotes Epithelial Tumor Cells Spreading and Regulates the Turnover of PKC? Activity under Mechanical Stimulation on the Elastomeric Substrates." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 36, Issue 4, pp. 1291-1304, (2015) (PubMed).

Details zu PKC alpha

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Andere Bezeichnung: PKC alpha

Hintergrund:

Synonyms: kinase PKC-alpha, KPCA, PKC III, PKC-A, PKC-alpha, PKC-III, PRKACA, PRKCA, Protein kinase C, alpha type

NCBI Gene Symbol: PRKCA

Molekulargewicht: 76 kDa

Gen-ID: 5578

OMIM: 176960

UniProt: P17252

Pathways: WNT Signalweg, T-Zell Rezeptor Signalweg, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling