CRISP2 Antikörper (AA 81-93)

Produktnummer ABIN1493872

Produktdetails anti-CRISP2 Antikörper

Target: CRISP2 (Cysteine-Rich Secretory Protein 2 (CRISP2))

Bindungsspezifität: AA 81-93

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CRISP2 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human CRISP2. Reported variants represent identical protein: NP_003287.1, NP_001135879.1, NP_001135889.1, NP_001135907.1, NP_001135880.1.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%) Monkey, Marmoset (92%) Rabbit (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-QHSDPEDRKTSTR from an internal region of human CRISP2 (NP_003287.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%), Monkey, Marmoset (92%), Rabbit (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:8000)

Usage: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: In transfected HEK293 transiently expressing Human CRISP2 (MYC and DYKDDDDK tagged), a band of approx. 35  kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.5-1 μg/mL. Preliminary experiments gave an approx 55  kDa band in Human Testis lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 27.3  kDa according to NP_003287.1. The 55  kDa band was successfully blocked by incubation with the immunizing peptide.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu CRISP2

Target: CRISP2 (Cysteine-Rich Secretory Protein 2 (CRISP2))

Andere Bezeichnung: CRISP2 / TSP1 (CRISP2 Produkte)

Synonyme: ct36 antikoerper, tpx1 antikoerper, tsp1 antikoerper, crisp antikoerper, gapdl5 antikoerper, crisp-2 antikoerper, CRISP3 antikoerper, CRISP2 antikoerper, CRISP-2 antikoerper, GAPDL5 antikoerper, Tpx-1 antikoerper, Tpx1 antikoerper, Crisp2l antikoerper, CT36 antikoerper, TPX1 antikoerper, TSP1 antikoerper, CRISP-3 antikoerper, CRISP-1 antikoerper, Crisp2 antikoerper, cysteine-rich secretory protein 2 L homeolog antikoerper, cysteine rich secretory protein 2 antikoerper, cysteine-rich secretory protein 2 antikoerper, crisp2.L antikoerper, CRISP2 antikoerper, Crisp2 antikoerper

Hintergrund: Name/Gene ID: CRISP2

Synonyms: CRISP2, CRISP-2, GAPDL5, Testis-specific protein TPX-1, TPX1, TSP1, Cancer/testis antigen 36, CT36

Gen-ID: 7180

NCBI Accession: NP_003287

UniProt: P16562