PPP1R9A Antikörper (AA 115-165) (HRP)

Produktnummer ABIN1412396

Der Kaninchen Polyklonal anti-PPP1R9A Antikörper wird verwendet zum Nachweis von PPP1R9A in Proben von Maus und Ratte. Er wurde validiert für ELISA, IHC (fro) und IHC (p).

Kurzübersicht für PPP1R9A Antikörper (AA 115-165) (HRP) (ABIN1412396)

Target: PPP1R9A (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 9A (PPP1R9A))

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PPP1R9A Antikörper ist konjugiert mit HRP

Applikation: ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PPP1R9A Antikörper (HRP)

Bindungsspezifität: AA 115-165

Kreuzreaktivität: Maus, Ratte

Homologie: Human,Dog,Cow,Sheep,Pig,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Neurabin 1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC-P 1:200-400
IHC-F 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu PPP1R9A

Target: PPP1R9A (Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 9A (PPP1R9A))

Andere Bezeichnung: Neurabin 1/PPP1R9A

Hintergrund:

Synonyms: FLJ20068, KIAA1222, NEB1_HUMAN, Neurabin I, Neurabin-1, Neurabin-I, Neurabin1, Neurabin-1, NeurabinI, Neurabin1, Neural tissue specic F actin binding protein I, Neural tissue-specic F-actin-binding protein I, NRB 1, NRB I, NRB1, NRBI, PPP1R9A, Protein phosphatase 1 regulatory inhibitor subunit 9A, Protein phosphatase 1 regulatory subunit 9A.

Background: Brain-specific neurabin I (neural tissue-specific F-actin binding protein I) is highly concentrated in the synapse of developed neurons, it localizes in the growth cone lamellipodia during neuronal development (1). Suppression of endogenous neurabin in rat hippocampal neurons inhibits neurite formation (1). Neurabin I recruits active PP1 via a PP1-docking sequence, mutation of the PP1-binding motif halts filopodia and restores stress fibers in neurabin I-expressing cells (2,3). Neurabin II (Spinophilin) is ubiquitously expressed but is abundantly expressed in brain (4). Neurabin II localizes to neuronal dentritic spines, which are the specialized protrusions from dendritic shafts that receive most of the excitatory input in the CNS (5). Neurabin II may regulate dendritic spine properties as neurabin II(-) mice have increased spine density during development in vitro and exhibit altered filopodial formation in cultured cells (5). Neurabin may also play a role in glutamatergic transmission as Neurabin II(-) mice exhibit reduced long-term depression and resistance to kainate-induced seizures and neronal apoptosis (5). Neurabin II complexes with the catalytic subunit of protein phosphatase-1 (PP1) in vitro thus modulating the activity of PP1 (4).