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RNA Polymerase 2 (AA 101-200) Antikörper (AbBy Fluor® 555)

Reaktivität: Maus WB, FACS, IF (cc), IF (p) Wirt: Kaninchen Polyclonal AbBy Fluor® 555
Produktnummer ABIN1397720
  • Target
    RNA Polymerase 2
    Bindungsspezifität
    • 16
    • 16
    • 15
    AA 101-200
    Reaktivität
    • 32
    • 16
    • 15
    Maus
    Wirt
    • 47
    Kaninchen
    Klonalität
    • 47
    Polyklonal
    Konjugat
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    AbBy Fluor® 555
    Applikation
    • 47
    • 41
    • 38
    • 38
    • 9
    • 9
    • 9
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
    Kreuzreaktivität
    Maus
    Homologie
    Human,Rat,Dog,Cow,Pig,Horse
    Aufreinigung
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human Pol II/RNA polymerase II
    Isotyp
    IgG
  • Applikationshinweise
    FCM 1:20-100
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 μg/μL
    Buffer
    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    Konservierungsmittel
    ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Target
    RNA Polymerase 2
    Hintergrund

    Synonyms: POLR2A, POLR2, DNA directed RNA polymerase II A, DNA-directed RNA polymerase II largest subunit, DNA-directed RNA polymerase II subunit A, RPB1_HUMAN.

    Background: RNA polymerase II (Pol II) is an enzyme that is composed of twelve subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.

    Gen-ID
    5430
    UniProt
    P24928
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