SPRY1 Antikörper (AA 221-319)

Produktnummer ABIN1386119

Produktdetails anti-SPRY1 Antikörper

Target: SPRY1 (Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))

Bindungsspezifität: AA 221-319

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SPRY1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Kreuzreaktivität: Human

Homologie: Mouse,Rat,Dog,Cow,Pig,Horse,Chicken

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Sprouty1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu SPRY1

Target: SPRY1 (Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))

Andere Bezeichnung: Sprouty1 (SPRY1 Produkte)

Synonyme: hSPRY1 antikoerper, SPRY1 antikoerper, LOC100223875 antikoerper, hspry1 antikoerper, xspry1 antikoerper, sprouty antikoerper, sprouty1 antikoerper, sprouty-1 antikoerper, Xtsprouty1 antikoerper, spry1a antikoerper, spry1b antikoerper, sprouty RTK signaling antagonist 1 antikoerper, sprouty RTK signaling antagonist 1 L homeolog antikoerper, sprouty RTK signaling antagonist 1 S homeolog antikoerper, sprouty homolog 1 (Drosophila) antikoerper, SPRY1 antikoerper, Spry1 antikoerper, spry1 antikoerper, spry1.L antikoerper, spry1.S antikoerper

Hintergrund:

Synonyms: Sprouty 1, Sprouty-1, hSPRY1, Protein sprouty homolog 1, Sprouty homolog 1 antagonist of FGF signaling, Sprouty homolog 1, Spry-1, Spry1, SPY1_HUMAN.

Background: Members of the Sprouty family (Sprouty 1-4) are inducible negative regulators of growth factors that act through tyrosine kinase receptors. Mammalian Sprouty homologs share a well-conserved cysteine-rich C-terminal domain with their Drosophila counterparts. Both Sprouty 1 and 2 are anchored to membranes by palmitoylation, associate with caveolin-1 in perinuclear and vesicular structures and are phosphorylated on Serine residues. Upon stimulation, a subset is recruited to the leading edge of the plasma membrane. Sprouty 2 can associate with c-Cbl, a down regulator of RTK signaling, and inhibits the activities of several growth factors. Sprouty 2 also functions as a negative regulator of embryonic lung morphogenesis and growth. The well-conserved C-terminus of Sprouty contains two domains which are necessary for Sprouty 2 co-localization with microtubules and translocation to membrane ruffles. In addition, the C-terminus is required for the inhibition of cell migration and proliferation. In conclusion, members of Sprouty inhibit FGF and VEGF-mediated cell proliferation, suggesting that they may regulate angiogenesis in normal and disease processes.

Pathways: EGFR Signaling Pathway, EGFR Downregulation