5-Methylcytosine Antikörper
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- Target
- 5-Methylcytosine
- Reaktivität
- Bitte anfragen
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Unkonjugiert
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Applikation
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Kreuzreaktivität
- Human
- Kreuzreaktivität (Details)
- 5 MethylCytosine
- Aufreinigung
- Purified by Protein A.
- Immunogen
- KLH conjugated 5 MethylCytosine
- Isotyp
- IgG
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- Applikationshinweise
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IHC-P 1:100-500
IF(IHC-P) 1:50-200 - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- 5-Methylcytosine
- Andere Bezeichnung
- 5 MethylCytosine
- Substanzklasse
- Chemical
- Hintergrund
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Synonyms: 5-Methyl Cytidine, 5 m C, 5 mC, 5 me C, 5 Me Cytidine, 5 MeCyd, 5 Methyl Cytidine, 5 MethylCytosine, 5-mC, 5-Me Cytidine, 5-Methyl-Cytosine, 5-MethylCytidine.
Background: Cytidine is a nucleoside formed by a cytosine attached to a ribose ring via a beta-N1-glycosidic bond. DNA is methylated on cytidines by DNA methylases (DNMTs)to generate 5-methylcytidine (5-mC), a potent epigenetics marker and regulator of gene expression. The reverse reaction (cytidine demethylation) starts with its oxidation to hydroxymethyl- (5-hmC), formyl- (5-fC), and carboxy- (5-caC) cytidine. Several enzymes, including the Tet family of proteins have been implicated in cytidine demethylation.
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