CD45RA/B Antikörper (Biotin)

Produktnummer ABIN133312

Produktdetails anti-CD45RA/B Antikörper (Biotin)

Target: CD45RA/B

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD45RA/B Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS)

Spezifität: Rat CD45RA/B

Aufreinigung: Ion exchange chromatography

Immunogen: Rat purified spleen L-CA . Fusion Partner: NSO/U . Donor: BALB/c Spleen

Klon: OX-33

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: This clone is reported to work with frozrn and paraffin sections (2).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 µg/10 cells

Lagerung: 4 °C

Details zu CD45RA/B

Target: CD45RA/B

Andere Bezeichnung: CD45RA/B (CD45RA/B Produkte)

Hintergrund: Rat Leukocyte Common Antigen has been shown to exist in different forms on different lymphoid cell types. Rat L-CA on thymocytes shows one main band at 180 kDa, T cells show 4 bands at 180, 190, 200 and 220 kDa. ABIN133312detects a subfraction of the 240 kDa rat L-CA band found only on B lymphocytes. This monoclonal antibody is of particular use in the investigation of the molecular and antigenic heterogeneity of rat L-CA, especially when used in conjunction with other OX antibodies. Four mouse monoclonal antibodies (MRC OX 1, 28, 29 and 30) react with all molecular forms of L-CA and fall into two sets that are non- competitive in binding to L-CA (MRC OX 1, 28, 29 and 30). The antigenic determinants seen by all these antibodies are lost when L-CA is reduced and alkylated. Three antibodies (MRC OX 22, 31, and 32) react selectively with B cells, T cytotoxic cells and about two thirds of T helper cells. OX 22 and OX 31 compete for binding but are non- competitive with OX 32. All of these antibodies bind to a sub-fraction of the 190, 200 and 220 kDa forms of T cell L-CA but not at all to the 180 kDa form of T cells or thymocytes. This clone is reported to work with frozrn and paraffin sections (2).