CD4 Antikörper

Produktnummer ABIN133174

Produktdetails anti-CD4 Antikörper

Target: CD4

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD4 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS)

Spezifität: Rat CD4

Aufreinigung: Purified from ascitic fluid via Protein G Chromatography

Immunogen: Rat thymocyte membrane . Fusion Partner: P3-NSI/1-Ag 4 . Donor: BALB/c spleen

Klon: W3-25

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: This clone is reported to work with frozen and paraffin sections (11) and functional testing (12).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 µg/10 cells

Buffer: Purified Ig buffered in PBS and 0.02% NaN (Purified from ascitic fluid via Protein G Chromatography).

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Referenzen für anti-CD4 Antikörper (ABIN133174)

Saravanan, Sareen, Abu-El-Rub, Ashour, Sequiera, Ammar, Gopinath, Shamaa, Sayed, Moudgil, Vadivelu, Dhingra: "Graphene Oxide-Gold Nanosheets Containing Chitosan Scaffold Improves Ventricular Contractility and Function After Implantation into Infarcted Heart." in: Scientific reports, Vol. 8, Issue 1, pp. 15069, (2019) (PubMed).

Details zu CD4

Target: CD4

Andere Bezeichnung: CD4 (CD4 Produkte)

Synonyme: cd4 antikoerper, L3T4 antikoerper, Ly-4 antikoerper, CD4mut antikoerper, W3/25 antikoerper, p55 antikoerper, CD4 molecule antikoerper, carcinoembryonic antigen-related cell adhesion molecule 5 antikoerper, T-cell surface glycoprotein CD4 antikoerper, CD4 antigen antikoerper, Cd4 molecule antikoerper, CD4 antikoerper, LOC100125525 antikoerper, cd4 antikoerper, Cd4 antikoerper

Hintergrund: Anti-rat T helper cell monoclonal antibody recognizes a determinant on the majority of thymocytes (90-95%), a subset of peripheral T cells and peritoneal macrophages. (1,2,3,10.) The antigen recognized by this antibody is a surface glycoprotein of Mr 48 000 - 52 000 and is the homologue of the human CD4 and the mouse L3/T4 antigen. ABIN133174labels the rat T helper subset, which mediates the helper activity for B and T cells, graft vs. host (GVH) reactivity and produces IL-2 in the mixed lymphocyte reaction (MLR). (2,4,6) Addition of ABIN133174to the MLR, inhibited proliferation and blocks the production of IL- 2. (4,5,6,9) T cells which mediate cytotoxicity and suppressor functions are not labelled. (Thus, cells labelled by this antibody are not labelled by MRC OX-8.) ABIN133174is invaluable for separating T cell subsets for functional studies and for labelling cells in tissue sections. It has been used in studying the role of T lymphocytes in graft rejections (7) and in studying the subsets of T cells in the rat which mediate graft vs. host disease. (8) This particular antibody is also one of three antibodies which labels T lymphocyte populations in the rat. These clones include W3/13 ABIN133174, which labels all T cells, as well as MRC OX-8 ABIN133174 and W3/25 ABIN133174 which label non-overlapping T cell subpopulations. These monoclonal antibodies used in concert are being employed extensively to investigate cellular aspects of the immune response in rats and prove to be useful as markers for functionally distinct subpopulations of lymphocytes. This clone is reported to work with frozen and paraffin sections (11) and functional testing (12).

Pathways: T-Zell Rezeptor Signalweg, Maintenance of Protein Location, CXCR4-mediated Signaling Events