RAF1 Antikörper (pSer259)
Kurzübersicht für RAF1 Antikörper (pSer259) (ABIN127169)
Target
Alle RAF1 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- pSer259
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Spezifität
- The antibody detects ~74 kDa c-Raf when phosphorylated at Ser259.
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Aufreinigung
- Affinity purified
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anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 31-130) antibody
RAF1 Reaktivität: Human ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated
anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 190-350) antibodyRAF1 Reaktivität: Human WB, IHC, IF, IP Wirt: Kaninchen Polyclonal unconjugated
anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 1-130) antibodyRAF1 Reaktivität: Human WB, ELISA, PLA, RNAi Wirt: Maus Monoclonal 1H4 unconjugated
anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibodyRAF1 Reaktivität: Human WB, ELISA, IHC, FACS Wirt: Maus Monoclonal 4G4 unconjugated
anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (pSer621) antibodyRAF1 Reaktivität: Human, Maus, Ratte WB, ELISA, IHC, IF, ICC Wirt: Kaninchen Polyclonal unconjugated
anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (pSer259) antibodyRAF1 Reaktivität: Human, Maus, Ratte WB, ELISA, IHC, IF, ICC Wirt: Kaninchen Polyclonal unconjugated
anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) antibodyKD Validated RAF1 Reaktivität: Human WB, FACS Wirt: Kaninchen Monoclonal 23GB2700 unconjugated Recombinant Antibody
anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (pSer338) antibodyRAF1 Reaktivität: Human, Maus, Ratte WB, ELISA, IF, ICC Wirt: Kaninchen Polyclonal unconjugated
anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 300-350) antibodyRAF1 Reaktivität: Human, Maus WB, FACS, IP Wirt: Kaninchen Monoclonal BLR289L unconjugated Recombinant Antibody
anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (AA 1-648) antibodyRAF1 Reaktivität: Human WB, ELISA, IHC, IF, IP Wirt: Kaninchen Polyclonal unconjugated
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Applikationshinweise
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Western Blot: 0.5-4 μg/mL. Immunohistochemistry: 40 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 0.5 mg/mL
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Buffer
- PBS, pH 7.2, containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal
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Konservierungsmittel
- Thimerosal (Merthiolate)
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Vorsichtsmaßnahmen
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handhabung
- Avoid repeated freezing and thawing.
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Lagerung
- -20 °C/-80 °C
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Informationen zur Lagerung
- Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.
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- RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
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Andere Bezeichnung
- RAF1
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Hintergrund
- C-Raf is a mitogen-activated protein kinase. Binding of Raf to 14-3-3 is required for c-Raf kinase activity, and also blocks the ability of phosphatase to inhibit c-Raf. 14-3-3 binds c-Raf at two phospho-serine residues: Ser259 and Ser621. Mutation of Ser259 to alanine constitutively activates Raf kinase activity, but mutation of Ser621 to alanine results in an inactive kinase.Synonyms: C-RAF, RAF proto-oncogene serine/threonine-protein kinase, Raf-1, cRaf
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Gen-ID
- 5894
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NCBI Accession
- NP_002871
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UniProt
- P04049
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Pathways
- MAPK Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling
Target
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