The polyclonal antibody reacts with Orf1 (FrpD), a 28 - 32 kDa Neisseria meningitidis outer membrane lipoprotein. Orf1 is highly conserved in a set of meningococcal strains representative of all serogroups and does not exhibit any similarity to known sequences of other organisms.
Kreuzreaktivität (Details)
Neisseria meningitidis
Aufreinigung
Purified by ligand affinity chromatography.
Reinheit
> 95 % (by SDS-PAGE)
Immunogen
Slices containing purified FrpD250-6xHis-tagged protein were excised from 10% SDS-PAGE gels, grinded, mixed with 1 mg/ml of aluminum hydroxide in PBS, and used to immunize a rabbit.
Western blotting: Recommended dilution 0.5-1 μg/mL, reducing conditions.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1 mg/mL
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Do not freeze.
Lagerung
4 °C
Informationen zur Lagerung
Store at 2-8°C. Do not freeze.
Prochazkova, Osicka, Linhartova, Halada, Sulc, Sebo: "The Neisseria meningitidis outer membrane lipoprotein FrpD binds the RTX protein FrpC." in: The Journal of biological chemistry, Vol. 280, Issue 5, pp. 3251-8, (2005) (PubMed).
Target
Neisseria Meningitidis Antigen Orf1
Andere Bezeichnung
Neisseria meningitidis antigen Orf1 / FrpD
Hintergrund
Orf1 (FrpD) is a Neisseria meningitidis outer membrane lipoprotein. Subcellular localization and [3H]palmitic acid labeling in Escherichia coli revealed that FrpD is synthesized with a type II signal peptide for export across the cytoplasmic membrane and is, upon processing to a lipoprotein, sorted to the outer bacterial membrane.,FrpD