CD43 Antikörper

Produktnummer ABIN120595

Produktdetails anti-CD43 Antikörper

Target: CD43 (SPN) (Sialophorin (SPN))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD43 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Functional Studies (Func)

Spezifität: The antibody recognizes human neuraminidase-sensitive epitope on CD43 (Leukosialin), a 95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. CD43 is expressed by platelets and at high levels on the surface of all leukocytes, it is negative on resting B lymphocytes and erythrocytes.

Aufreinigung: Protein-A affinity chromatography

Reinheit: > 95 % pure (by SDS-PAGE)

Immunogen: Human T lymphocytes

Klon: MEM-59

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blotting (non reducing conditions). Immunoprecipitation. Flow cytometry. Immunohistochemistry on paraffin sections. Functional applications (activates T lymphocytes. CD43 antibody ABIN120595 induces apoptosis in hemopoietic progenitor cells).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody at 2-8 °C for up to one month or at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Haltbarkeit: 12 months

Referenzen für anti-CD43 Antikörper (ABIN120595)

Kosina, Plisek, Dostal, Moravkova, Cermak, Preis, Lukes, Kracmarova, Krausova: "[Invasive streptococcal infections]" in: Klinická mikrobiologie a infekc?ní lékar?ství, Vol. 13, Issue 6, pp. 220-4, (2008) (PubMed).

Details zu CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Andere Bezeichnung: CD43 / Leukosialin (SPN Produkte)

Synonyme: CD43 antikoerper, GALGP antikoerper, GPL115 antikoerper, LSN antikoerper, A630014B01Rik antikoerper, Cd43 antikoerper, Galgp antikoerper, Ly-48 antikoerper, Ly48 antikoerper, Lsn antikoerper, Lsn1 antikoerper, gpL115 antikoerper, sialophorin antikoerper, SPN antikoerper, Spn antikoerper

Hintergrund: CD43 is a transmembrane mucin-like protein with high negative charge, expressed on the surface of most hematopoietic cells. CD43 contributes to a repulsive barrier that interferes with cellular adhesion, however, in certain cases also promotes leukocyte aggregation. By interaction with actin-binding proteins ezrin and moesin CD43 plays a regulatory role in remodeling T-cell morphology and regulates cell-cell interactions during lymphocyte traffic. CD43 signaling both enhances LFA-1 adhesiveness and counteracts LFA-1 induction via other receptors. Expression of CD43 causes induction of functionally active tumour suppressor p53 protein, but in case of p53 and ARF defficiency CD43 promotes tumour proliferation and viability. It appears to be an important modulator of leukocyte functions.Synonyms: Galactoglycoprotein, Leukocyte sialoglycoprotein, SPN, Sialophorin

Gen-ID: 6693

UniProt: P16150

Pathways: Regulation of Leukocyte Mediated Immunity