This antibody recognizes the Human high affinity Fc receptor for IgE (Fc epsilon R1), which plays a central role in the IgE-mediated allergic response. Clone 9E1 specifically recognizes the extracellular D1 domain of the Fc epsilon R1 alpha chain.
Aufreinigung
Affinity Chromatography on Protein G
Immunogen
CCR7 rat basophilic leukaemia (RBL)-2H3 transfectants. Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0-Ag14 myeloma cell line.
Verified
FCER1A
Reaktivität: Human
WB, ELISA
Wirt: Ziege
Polyclonal
unconjugated
Applikationshinweise
Flow Cytometry: Use 10 μL of 1/50-1/50 diluted antibody to label 106 cells in 100 μL. ELISA. Immunoprecipitation. Western Blot. Immunofluorescence. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS, 0.09 % Sodium Azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target
Fc epsilon RI/FCER1A (FCER1A)
(Fc Fragment of IgE Receptor Ia (FCER1A))
FCER1A antikoerper, FCE1A antikoerper, FcERI antikoerper, Iger01 antikoerper, RATIGER01 antikoerper, Fce1a antikoerper, Fcr-5 antikoerper, fcepsilonri antikoerper, Fc fragment of IgE receptor Ia antikoerper, Fc receptor, IgE, high affinity I, alpha polypeptide antikoerper, FCER1A antikoerper, Fcer1a antikoerper
Hintergrund
Fc epsilon R1 consists of four subunits, a high affinity IgE binding alpha subunit, a beta chain and two di-sulphide linked gamma subunits. Fc epsilon R1 is primarily expressed on mast cells and basophils but expression of Fc epsilon R1 has also been reported on monocytes, Langerhans cells and dendritic cells from patients with atopic diseases.Synonyms: FCE1A, Fc-epsilon RI-alpha, FcERI, High affinity immunoglobulin epsilon receptor subunit alpha, IgE Fc receptor subunit alpha