ITGA4 Antikörper

Produktnummer ABIN118228

Produktdetails anti-ITGA4 Antikörper

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reaktivität: Human, Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser ITGA4 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: This antibody recognises alpha 4 integrin (CD49d). Clone PS/2 has also been reported to block the binding of CD49d to its ligands. We recommend the use of SM037LE for this purpose.

Aufreinigung: Affinity chromatography on Protein G.

Klon: PS-2

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: Use 10 μL of 1/50-1/100 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Andere Bezeichnung: CD49d / ITGA4 (ITGA4 Produkte)

Synonyme: CD49D antikoerper, IA4 antikoerper, LOC100219853 antikoerper, integrin subunit alpha 4 antikoerper, integrin alpha 4 antikoerper, integrin subunit alpha 4 S homeolog antikoerper, ITGA4 antikoerper, Itga4 antikoerper, itga4.S antikoerper

Hintergrund: Integrin α4 (also called CD49d) is a ~150 kDa protein that possesses a large extracellular domain involved in ligand binding, a single transmembrane domain, and an intracellular regulatory domain possessing multiple sites for phosphorylation. Integrin α4 forms heterodimers with integrins β1 and β7. Integrin α4 is expressed on leukocytes and leukocyte precursors, neural crest cells, and developing skeletal muscles and is essential for embryogenesis, hematopoiesis, and immune responses. The presence of integrin α4 promotes cell migration and inhibits cell spreading and contractility. Integrin α4 function has been implicated in the pathogenesis of multiple diseases including asthma, rheumatoid arthritis, Crohn's disease, ulcerative colitis, hepatitis C, and multiple sclerosis, and therefore, modulation of integrin α4 function has become an important target for drug discovery. Phosphorylation of the serine 988 residue on the integrin α4 cytoplasmic tail inhibits its interaction with the focal adhesion complex-associated protein paxillin, and modulates its effects on cell motility and spreading.Synonyms: CD49 antigen-like family member D, Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4

Gen-ID: 3676

NCBI Accession: NP_000876

UniProt: P13612

Pathways: Integrin Complex