Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. This product has been assayed against 1.0 g of Bovine IgG in a standardcapture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1:11,000 to 1:45,000 of the reconstitution concentration is suggestedfor this product.
Other applications not tested. Otimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore with 1.0 mL of deionized water (or equivalent).
Konzentration
2.0 mg/mL
Buffer
0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 contaning 0.01 % (w/v) Thimerisol as preservative and 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer.
Konservierungsmittel
Thimerosal (Merthiolate)
Vorsichtsmaßnahmen
Biohazard Informations: This product contains thimerosal which is hazardous.
Handhabung
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.