Ziege anti-Rind (Kuh) IgG (Whole Molecule) Antikörper (Biotin)

Details zu Produkt Nr. ABIN118075, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Whole Molecule
4782
2533
1961
1067
1022
861
306
292
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
Reaktivität
Rind (Kuh)
2897
2761
2297
1676
1453
790
626
453
395
387
359
346
298
296
221
138
135
130
41
31
29
24
16
11
10
8
7
6
5
5
4
4
4
3
3
3
3
3
3
3
2
2
1
1
1
1
1
Wirt
Ziege
6785
4909
1788
923
609
392
91
68
55
18
8
5
5
5
2
1
1
Klonalität
Polyklonal
Konjugat
Biotin
2268
1903
1784
1360
769
686
531
294
268
227
226
201
158
141
129
110
79
75
69
55
52
48
48
45
40
39
37
35
35
29
28
28
27
27
27
27
27
27
22
19
19
19
19
17
15
15
15
15
15
15
14
12
12
11
10
10
10
9
9
9
9
9
8
8
8
8
6
6
6
6
6
5
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Applikation
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Bovine IgG whole molecule.
Produktmerkmale Molar Ratio: 10-20 BAC molecules per Goat IgG molecule.
Reinigung Immunoaffinity chromatography.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Hintergrund Synonyms: Bovine Immunoglobulin G
Applikationshinweise Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. This product has been assayed against 1.0 g of Bovine IgG in a standardcapture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1:11,000 to 1:45,000 of the reconstitution concentration is suggestedfor this product.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Rekonstitution Restore with 1.0 mL of deionized water (or equivalent).
Konzentration 2.0 mg/mL
Buffer 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 contaning 0.01 % (w/v) Thimerisol as preservative and 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer.
Konservierungsmittel Thimerosal (Merthiolate)
Vorsichtsmaßnahmen Biohazard Informations: This product contains thimerosal which is hazardous.
Handhabung Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Allgemeine Veröffentlichungen Bayer, Wilchek: "Protein biotinylation." in: Methods in enzymology, Vol. 184, pp. 138-60, 1990 (PubMed).