TLK1 Antikörper (Isoform 1, pSer695)

Produktnummer ABIN117936

Produktdetails anti-TLK1 Antikörper

Target: TLK1 (Tousled-Like Kinase 1 (TLK1))

Bindungsspezifität: AA 737-751, Isoform 1, pSer695

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TLK1 Antikörper ist unkonjugiert

Applikation: Enzyme Immunoassay (EIA)

Sequenz: Human Tlk-1 (isoform 1), 766 aa, predicted MW 86.7 kDa: 1 msvqsssgsl egppswsqls tsptpgsaaa arsllnhtpp sgrpregamd elhsldprrq 61 ellearftgv asgstgstgs csvgakastn nessnhsfgs lgslsdkese tpekkqsess 121 rgrkrkaenq nessqgksig grghkisdyf eyqggngssp vrgippairs pqnshshstp 181 sssvrpnsps ptalafgdhp ivqpkqlsfk iiqtdltmlk laalesnkiq dlekkegrid 241 dllrancdlr rqideqqkll ekykerlnkc ismskkllie kstqeklssr eksmqdrlrl 301 ghfttvrhga sfteqwtdgf afqnlvkqqe wvnqqredie rqrkllakrk pptannsqap 361 stnsepkqrk nkavngaend pfvrpnlpql ltlaeyheqe eifklrlghl kkeeaeiqae 421 lerlervrnl hirelkrinn ednsqfkdhp tlnerylllh llgrggfsev ykafdlyeqr 481 yaavkihqln kswrdekken yhkhacreyr ihkeldhpri vklydyfsld tdtfctvley 541 cegndldfyl kqhklmseke arsivmqivn alrylneikp piihydlkpg nillvdgtac 601 geikitdfgl skimdddsyg vdgmdltsqg agtywylppe cfvvgkeppk isnkvdvwsv 661 gviffqclyg rkpfghnqsq qdilqentil katevqfpvk pvvsseakaf irrclayrke 721 drfdvhqlan dpyllphmrr snssgnlhma gltasptpps ssiity

Aufreinigung: Immunoaffinity chromatography.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 737-751 of human Tlk1 (Tousled-like kinase) (isoform 1) protein.

Anwendungsinformationen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA (1: 10,000-1: 50,000) and byWestern blot (1: 200- 1: 2,000). Specific conditions for reactivity should be optimized by the end user. Expect a bandapproximately 86 kDa, 89 kDa or 64 kDa in size corresponding to isoforms of TLk-1 byWestern blotting in the appropriate cell lysate or extract. This antibody reacts with bothphosphorylated and non-phosphorylated forms of Tlk-1 at the S743 residue (isoform 1) ofthe protein.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.6 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. For extended storage aliquot contents and freeze at -20 °C or below.

Details zu TLK1

Target: TLK1 (Tousled-Like Kinase 1 (TLK1))

Andere Bezeichnung: TLK1 (TLK1 Produkte)

Synonyme: PKU-beta antikoerper, 4930545J15Rik antikoerper, fe11e12 antikoerper, si:dkey-251e16.4 antikoerper, tlk1 antikoerper, wu:fe11e12 antikoerper, TLK1 antikoerper, fb76h12 antikoerper, si:dkey-88m1.1 antikoerper, wu:fb76h12 antikoerper, DKFZp459L163 antikoerper, tousled like kinase 1 antikoerper, tousled-like kinase 1 antikoerper, tousled-like kinase 1b antikoerper, tousled like kinase 1 like antikoerper, serine/threonine-protein kinase TOUSLED antikoerper, tousled-like kinase 1a antikoerper, Serine/threonine-protein kinase tousled-like 1 antikoerper, TLK1 antikoerper, Tlk1 antikoerper, tlk1b antikoerper, TLK1L antikoerper, LOC542181 antikoerper, tlk1a antikoerper, tlk1 antikoerper, tlk-1 antikoerper

Hintergrund: Tlk-1 (also known as tousled-like kinase, serine/threonine-protein kinase tousled-like 1 and PKU-beta) is a target for Chk1 upon induction of DNA-damage. Tousled-like kinases, first described in Arabadopsis, are nuclear serine/threonine kinases that are potentially involved in the regulation of chromatin assembly. Tlk-1 is rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. This is cell cycle checkpoint and ATM-pathway dependent. Tlk-1 heterodimerizes with Tlk-2. Multiple isoforms of the protein have been detected. Mature Tlk-1 is reported to have an apparent molecular weight of ~86 kDa, ~89 kDa and ~64 kDa corresponding to isoforms 1, 2 and 3 respectively.Synonyms: KIAA0137, PKU-beta, Serine/threonine-protein kinase tousled-like 1, Tousled-like kinase 1

NCBI Accession: NP_036422

UniProt: Q9UKI8