Phospholipase C gamma 2 Antikörper (pTyr759) (PE)

Produktnummer ABIN1177149

Dieses Anti-Phospholipase C gamma 2-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Phospholipase C gamma 2 in ICS. Geeignet für Human, Ratte und Maus. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für Phospholipase C gamma 2 Antikörper (pTyr759) (PE) (ABIN1177149)

Target: Phospholipase C gamma 2 (PLCG2)

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Phospholipase C gamma 2 Antikörper ist konjugiert mit PE

Applikation: Intracellular Staining (ICS)

Klon: K86-689-37

Produktdetails anti-Phospholipase C gamma 2 Antikörper (PE)

Bindungsspezifität: pTyr759

Marke: BD Phosflow™

Aufreinigung: Purified from tissue culture supernatant or ascites by affinity chromatography

Immunogen: Phosphorylated Human PLC-gamma2 Peptide

Isotyp: IgG1 kappa

Anwendungsinformationen

Probenmenge: 20 μL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Referenzen für anti-Phospholipase C gamma 2 Antikörper (PE) (ABIN1177149)

Kim, Sekiya, Poulin, Bae, Rhee: "Mechanism of B-cell receptor-induced phosphorylation and activation of phospholipase C-gamma2." in: Molecular and cellular biology, Vol. 24, Issue 22, pp. 9986-99, (2004) (PubMed).

Details zu Phospholipase C gamma 2

Target: Phospholipase C gamma 2 (PLCG2)

Andere Bezeichnung: PLC-gamma 2

Hintergrund: The Phospholipase C (PLC) isozymes hydrolyze phosphatidyl inositol biphosphate to inositol triphosphate and diacylglycerol. The former causes release of calcium from the endoplasmic reticulum, while the latter is an activator of Protein Kinase C. Within the PLC family, PLC-gamma is the only member that contains SH2 and SH3 domains. These domains enable it to interact with protein tyrosine kinases and become enzymatically activated via phosphorylation. It exists as two isoforms: PLC-g1, which is ubiquitously expressed, and PLC-g2, which is found primarily in the hematopoietic system. PLC-g2-null mice exhibit impaired B lymphocyte maturation and defects in Fc receptor functions. Phosphorylation of PLC-g2 at tyrosines 753 and 759 (Y759) is required for activation of PLC-g2 enzyme activity. PLC-g2 phosphorylation at Y759 can be induced by stimulation of the B cell receptor in Ramos cells, the collagen receptor in platelets, and the T cell receptor in Jurkat cells, and it occurs downstream of Btk and BLNK in the signaling cascade of activated B cells. The K86-689.37.73 antibody recognizes PLC-g2 phosphorylated at Y759 in the SH2-SH3 linker region.

Pathways: RTK Signalweg, WNT Signalweg, Fc-epsilon Rezeptor Signalübertragung, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades, VEGF Signaling, BCR Signaling