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EGFR2 Antikörper (AA 376-578) (FITC)

EGFR2 Reaktivität: Human IHC, WB, IF, ICC Wirt: Kaninchen Polyclonal FITC
Produktnummer ABIN1175126
  • Target Alle EGFR2 Antikörper anzeigen
    EGFR2 (Epidermal Growth Factor Receptor 2 (EGFR2))
    Bindungsspezifität
    AA 376-578
    Reaktivität
    • 4
    • 3
    Human
    Wirt
    • 6
    • 1
    Kaninchen
    Klonalität
    • 6
    • 1
    Polyklonal
    Konjugat
    • 4
    • 2
    • 1
    Dieser EGFR2 Antikörper ist konjugiert mit FITC
    Applikation
    • 7
    • 7
    • 4
    • 2
    • 2
    • 1
    • 1
    Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Sequenz
    MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS- FLPES FDGDPASNTA PLQPEQLQVF ETLEEITGYL YISAWPDSLP DLSVFQNLQV IRGRILHNGA YSLTLQGLGI SWLGLRSLRE LGSGLALIHH NTHLCFVHTV PWDQLFRNPH QALLHTANRP EDECVGEGLA CHQLCARGHC WGPGPTQCVN CSQFLRGQEC VEECRVLQGL PREYVNARHC LPCHPECQPQ NGSVTCFG
    Spezifität
    It has been selected for its ability to recognize EGFR2 in immunohistochemical staining and Western blotting.
    Aufreinigung
    Affinity Chromatography
    Immunogen
    Recombinant EGFR2 expressed in E.coli.
    The antibody is a rabbit polyclonal antibody raised against EGFR2 conjugated to fitc.
    Isotyp
    IgG
  • Applikationshinweise
    Western blotting: 1:100-400
    Immunocytochemistry in formalin fixed cells: 1:100-500
    Immunohistochemistry in formalin fixed frozen section: 1:100-500
    Immunohistochemistry in paraffin section: 1:50-200
    Enzyme-linked Immunosorbent Assay: 1:100-200
    Optimal working dilutions must be determined by end user.
    Kommentare

    Content: The quality control contains recombinant EGFR2 (Phe376~Gly578) disposed in loading buffer.
    Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
    Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
    Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handhabung
    Avoid repeated freeze/thaw cycles
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
    Haltbarkeit
    12 months
  • Target
    EGFR2 (Epidermal Growth Factor Receptor 2 (EGFR2))
    Abstract
    EGFR2 Produkte
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