Ziege anti-Human IgA, IgG, IgM (Whole Molecule) Antikörper (Biotin) - Preadsorbed

Produktnummer ABIN117310

Produktdetails

Target: IgA, IgG, IgM

Bindungsspezifität: Whole Molecule

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktmerkmale: Molar Ratio: 10-20 BAC molecules per Goat IgG molecule.

Aufreinigung: Preadsorption: Solid phase absorption

Immunogen: Human IgG, IgA and IgM whole molecules

Anwendungsinformationen

Applikationshinweise: Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as otherantibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. Recommended Dilutions: This product has been assayed against 1.0 μg of Human IgG in a standard capture ELISAusing Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid) as a substrate for 30 minutes at roomtemperature. A working dilution of 1:20,000 to 1:80,000 of the reconstitutionconcentration is suggested for this product.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1.0 mL deionized water or equivalent.

Konzentration: 2.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, 10 mg/mL BSA (IgG and Protease free)

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.

Antigendetails

Target: IgA, IgG, IgM

Abstract: IgA, IgG, IgM Produkte

Substanzklasse: Antibody

Hintergrund: Synonyms: Human IgA, Human IgG, Human IgM