MDH1 Antikörper

Produktnummer ABIN116777

Produktdetails anti-MDH1 Antikörper

Target: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Reaktivität: Schwein

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser MDH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Malate Dehydrogenase [Pig Heart]. Cross reactivity against Malate Dehydrogenase from other tissues and species may occur but have not been specifically determined.

Aufreinigung: Prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Malate Dehydrogenase [Pig Heart].

Anwendungsinformationen

Applikationshinweise: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of MalateDehydrogenase [Pig Heart] in a standard sandwich ELISA using Peroxidase conjugatedAffinity Purified anti-Sheep IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 3,000 to 1: 12,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with 2.0 mL of deionized water (or equivalent).

Konzentration: 80.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Details zu MDH1

Target: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Andere Bezeichnung: MDHA (MDH1 Produkte)

Synonyme: MDH-s antikoerper, MDHA antikoerper, MGC:1375 antikoerper, MOR2 antikoerper, ODS1 antikoerper, B230377B03Rik antikoerper, D17921 antikoerper, Mor-2 antikoerper, Mor2 antikoerper, MDL1 antikoerper, Mdhl antikoerper, MDH antikoerper, mdh-s antikoerper, mdha antikoerper, mor2 antikoerper, MDH-1 antikoerper, Mdh antikoerper, Mdh-1 antikoerper, Mdh2 antikoerper, MdhD antikoerper, cMdh antikoerper, sMdh antikoerper, malate dehydrogenase 1 antikoerper, malic enzyme 2 antikoerper, malate dehydrogenase 1, NAD (soluble) antikoerper, malate dehydrogenase 1 S homeolog antikoerper, malate dehydrogenase, cytoplasmic antikoerper, Malate dehydrogenase 1 antikoerper, MDH1 antikoerper, ME2 antikoerper, Mdh1 antikoerper, mdh1.S antikoerper, LOC100280767 antikoerper

Hintergrund: Malate dehydrogenase catalyzes the interconversion of L-malate and oxaloacetate using nicotinamide adenine dinucleotide (NAD) as a coenzyme. Malate dehydrogenase is found in all eukaryotic cells as two isozymes: mitochondrial (m-MDH) and cytoplasmic (soluble, s-MDH). Prokaryotes contain only a single form. The two isozymes, both consisting of two very similar subunits of about 35kD and having similar enzymatic activity appear as different proteins. There is structural similarity of mitochondrial malate dehydrogenase to L-3-hydroxyacyl CoA dehydrogenase and the cytoplasmic malate dehydrogenase to lactate dehydrogenase.Synonyms: MDH1, Malate dehydrogenase, cytoplasmic

Gen-ID: 396894

NCBI Accession: NP_999039

UniProt: P11708