Glycerol Kinase Antikörper

Produktnummer ABIN116737

Produktdetails anti-Glycerol Kinase Antikörper

Target: Glycerol Kinase (GK)

Reaktivität: Cellulomonas

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Glycerol Kinase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Glycerol Kinase [Cellulomonas species]. Cross reactivity against Glycerol Kinase from other sources is unknown.

Aufreinigung: This product was prepared from monospecific antiserum by a delipidation and defibrination.

Immunogen: Glycerol Kinase [Cellulomonas species]

Anwendungsinformationen

Applikationshinweise: Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended dilution: This product has been assayed against 1.0 μg of Glycerol Kinase [Celullomonas species] ina standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG[H&L] (Rabbit) and (ABTS(2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid])as asubstrate for 30 minutes at room temperature. A working dilution of 1: 1,000 to 1: 2,000 of the reconstitution concentration is suggested forthis product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with 2.0 mL of deionized water or equivalent.

Konzentration: 90 mg/mL (by refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Dilute only prior to immediate use

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 2-8 °C. DO NOT FREEZE. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Details zu Glycerol Kinase

Target: Glycerol Kinase (GK)

Andere Bezeichnung: Glycerol Kinase (GK Produkte)

Synonyme: GK1 antikoerper, GKD antikoerper, D930012N15Rik antikoerper, GK antikoerper, CG18374 antikoerper, Dmel\\CG18374 antikoerper, dGyk antikoerper, ASTP antikoerper, Gyk antikoerper, gk1 antikoerper, gk2 antikoerper, gkd antikoerper, gyk antikoerper, Gk antikoerper, BmGK antikoerper, DKFZp469P1225 antikoerper, glycerol kinase antikoerper, Glycerol kinase 1 antikoerper, glycerol kinase S homeolog antikoerper, putative glycerol kinase 3 antikoerper, ATP:glycerol 3-phosphotransferase; glycerokinase; GK antikoerper, Glycerol kinase antikoerper, Fanconi anemia core complex associated protein 100 antikoerper, GK antikoerper, Gk antikoerper, Gk1 antikoerper, gk.S antikoerper, LOC705001 antikoerper, glpK antikoerper, Spico_1557 antikoerper, Trebr_0397 antikoerper, Halhy_5315 antikoerper, gk antikoerper, FAAP100 antikoerper

Hintergrund: Glycerol kinase catalyzes the formation of glycerol 3 phosphate from ATP and glycerol. Dihydroxyacetone and L glyceraldehyde can also act as acceptors, UTP and, in the case of the yeast enzyme, ITP and GTP can act as donors. It provides a way for glycerol derived from fats or glycerides to enter the glycolytic pathway. NB - Glycerol kinase obtained from Cellulomonas spp has an observed molecular weight of 128 kDa.Synonyms: ATP:glycerol 3-phosphotransferase, GK, GKD, Glycerokinase

Gen-ID: 2710

NCBI Accession: NP_000158

UniProt: P32189