Intestinal Alkaline Phosphatase Antikörper

Produktnummer ABIN116604

Produktdetails anti-Intestinal Alkaline Phosphatase Antikörper

Target: Intestinal Alkaline Phosphatase (ALPI)

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Intestinal Alkaline Phosphatase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Aufreinigung: Prepared from monospecific antiserum by a Delipidation and Defibrination.

Immunogen: Alkaline Phosphatase [Calf intestine].

Anwendungsinformationen

Applikationshinweise: Suitable for immunoblotting (western or dot blot), ELISA, immunoprecipitation and mostimmunological methods requiring high titer and specificity. This product has been assayedagainst 1.0 μg of Alkaline Phosphatase [Calf intestine] in a standard sandwich ELISA usingPeroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 2,000 to 1: 8,000 of the reconstitutionconcentration is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: Restore with 2.0 mL of deionized water (or equivalent).

Konzentration: 85.0 mg/mL (by Refractometry)

Buffer: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 without preservatives.

Konservierungsmittel: Without preservative

Handhabung: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.

Details zu Intestinal Alkaline Phosphatase

Target: Intestinal Alkaline Phosphatase (ALPI)

Andere Bezeichnung: Alkaline Phosphatase / ALPI / IAP (ALPI Produkte)

Synonyme: IAP antikoerper, Alp1 antikoerper, S51097 antikoerper, 2010001C14Rik antikoerper, Akp6 antikoerper, gIAP antikoerper, alpi antikoerper, im:7149588 antikoerper, si:ch211-262h13.1 antikoerper, wu:fa95f04 antikoerper, zgc:110794 antikoerper, ALPI antikoerper, Alpi antikoerper, alkaline phosphatase, intestinal antikoerper, alkaline phosphatase, intestinal, tandem duplicate 1 antikoerper, intestinal-type alkaline phosphatase antikoerper, ALPI antikoerper, Alpi antikoerper, alpi.1 antikoerper, LOC100724954 antikoerper

Hintergrund: Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH . Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.Synonyms: Intestinal alkaline phosphatase

Gen-ID: 9913

UniProt: P19111