Nerve Growth Factor Antikörper

Produktnummer ABIN116191

Produktdetails anti-Nerve Growth Factor Antikörper

Target: Nerve Growth Factor (NGF)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Nerve Growth Factor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Functional Studies (Func), Enzyme Immunoassay (EIA)

Spezifität: Specific for Human beta NGF (Nerve Growth Factor). Other species not tested.

Aufreinigung: Affinity Chromatography employing immobilized Human beta-NGF matrix

Immunogen: Highly pure (>98%) E.coli derived recombinant Human beta-NGF.

Anwendungsinformationen

Applikationshinweise: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhuman beta NGF (1 ng/mL), a concentration of 0.05 - 0.08 μg/mL of this antibody isrequired. ELISA: To detect human beta NGF by direct ELISA (using 100l/well antibody solution) aconcentration of at least 0.5g/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2 - 0.4 ng/well of recombinant human beta NGF. Western Blot: To detect human beta NGF by Western Blot analysis this antibody can beused at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant human beta NGF is 1.5 - 3.0 ng/lane, undereither reducing or non-reducing conditions. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Details zu Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Andere Bezeichnung: NGF (NGF Produkte)

Synonyme: Beta-NGF antikoerper, HSAN5 antikoerper, NGFB antikoerper, Ngfb antikoerper, ngfb antikoerper, ns:zft386 antikoerper, xx:zft386 antikoerper, zgc:109730 antikoerper, beta-NGF antikoerper, hsan5 antikoerper, beta-ngf antikoerper, NGF antikoerper, ngf antikoerper, nerve growth factor antikoerper, nerve growth factor b (beta polypeptide) antikoerper, nerve growth factor S homeolog antikoerper, NGF antikoerper, Ngf antikoerper, ngfb antikoerper, ngf antikoerper, ngf.S antikoerper

Hintergrund: Nerve growth factor (NGF) is one of a family of neurotrophins that induce the survival and proliferation of neurons. In cell culture, NGF induces the formation of neurite projections and, in vivo, may stimulate the innervation of tissues. NGF plays a role in the repair, regeneration, and protection of neurons, and as such could serve as a therapeutic agent in neurodegenerative conditions such as Alzheimer's disease. NGF enhances survival, growth, neurotransmitter biosynthesis of sympathetic and sensory neurons, neurotrophic factor, cutaneous innervation, growth, differentiation and survival of B lymphocytes. It also has a possible role in allergy and tissue repair. NGF is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. NGF is upregulated by glutamate, vitamin D3, IL6, FGF basic, astrocyte specific IL1, TNF alpha, PDGF and TGF beta. It is downregulated by GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta.Synonyms: Beta-NGF, Beta-nerve growth factor, NGFB

Gen-ID: 4803

NCBI Accession: NP_002497

UniProt: P01138

Pathways: Regulation of Cell Size