Ziege anti-Maus IgG2b (Heavy Chain) Antikörper (Atto 550) - Preadsorbed
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- Target Alle IgG2b Produkte
- IgG2b
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Bindungsspezifität
- Heavy Chain
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Reaktivität
- Maus
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Atto 550
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Applikation
- Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- Spezifität
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Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2b.
Specificity was confirmed by ELISA at less than 0.5 % of target signal. - Kreuzreaktivität
- Maus
- Produktmerkmale
- ATTO Dye Conjugated Secondary Antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
- Aufreinigung
- Preadsorption: Solid phase absorption
- Immunogen
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Immunogen: Mouse IgG2b heavy chain
Immunogen Type: Native Protein
- Isotyp
- IgG2b
- Labeling Ratio
- 1.6
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- Applikationshinweise
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Immunohistochemistry Dilution: >1:5,000
Application Note: Anti-Mouse IgG2b secondary antibody is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG2b antibody based assays. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation. ATTO Dye Conjugated Secondary Antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: >1:20,000
Flow Cytometry Dilution: 1:500 - 1:2,500
Western Blot Dilution: >1:10,000
IF Microscopy Dilution: >1:5,000
- Kommentare
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The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
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Reconstitution Volume: 500 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Konzentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
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Avoid cycles of freezing and thawing.
Product is photosensitive and should be protected from light. - Lagerung
- RT,4 °C,-20 °C
- Haltbarkeit
- 12 months
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- Target
- IgG2b
- Abstract
- IgG2b Produkte
- Substanzklasse
- Antibody
- Hintergrund
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Synonyms: goat anti-MOUSE IgG2b Antibody ATTO550 Conjugation, goat anti-Mouse IgG2b (gamma 2b) ATTO 550 Conjugated Antibody
Background: Anti-Mouse IgG2b ATTO 550 Antibody generated in goat detects reactivity to Mouse IgG2b (Gamma 2b chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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