mEOS2 Antikörper

Produktnummer ABIN1110898

Produktdetails

Target: mEOS2

Reaktivität: Lobophyllia hemprichii

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: ELISA

Spezifität: This antibody binds specifically to mEos2 GFP.

Aufreinigung: Affinity Purified

Reinheit: The IgG fraction of tissue culture supernatant was purified by Protein A / G affinity chromatography.

Immunogen: The immunogen was recombinant mEos2 GFP. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen.

Klon: 12C11-C8

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: For ELISA we recommend a work dilution of 1: 64,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the antibody with distilled or de-ionized water to a final concentration of 1 mg/ml.

Buffer: Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives.

Informationen zur Lagerung: Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can also be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for at least 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 30 days at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.

Referenzen

Zhang, Chang, Zhang, Yu, Wu, Ji, Chen, Liu, Lu, Liu, Zhang, Xu, Xu: "Rational design of true monomeric and bright photoactivatable fluorescent proteins." in: Nature methods, Vol. 9, Issue 7, pp. 727-9, (2012) (PubMed).

Antigendetails

Target: mEOS2

Hintergrund: Monomeric (m)Eos2 is an engineered photoactivatable fluorescent protein widely used for superresolution microscopy. An optical highlighter isolated from another stony coral (Lobophyllia hemprichii), the tetrameric EosFP (named after the goddess of dawn in Greek mythology), behaves similarly to Kaede and the other variants described above. EosFP emits bright green fluorescence at 516 nanometers, and can be photoconverted to orange-red (581 nanometers) fluorescence when illuminated at near-ultraviolet wavelengths. Random and site-directed mutagenesis of tetrameric EosFP was used to generate two dimers and a true monomeric protein named mEosFP.