14-3-3 Pan Antikörper

Produktnummer ABIN1105134

Produktdetails

Target: 14-3-3 Pan

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB)

Kreuzreaktivität (Details): Species reactivity (tested):Human, mouse, rat

Aufreinigung: Protein A agarose

Immunogen: Recombinant human 14-3-3beta

Klon: 3C8

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 50 % glycerol, pH 7.2, containing no preservatives

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store (in aliquots) at -20 °C.

Antigendetails

Target: 14-3-3 Pan

Andere Bezeichnung: 14-3-3 Protein Pan

Hintergrund: 14-3-3 was first described as an abundant acidic protein in brain composing 1 % of the total soluble protein. The 14-3-3 family of proteins are expressed in all eukaryotic cells. There are at least seven highly conserved isoforms encoded by different gene products. These proteins have molecular weights of 29,000-32,000 and bind numerous cytoplasmic and nuclear signaling molecules including signaling molecules such as Raf, protein kinase C, p130Cas, BAD, and phosphatidylinositol 3-kinase. Interaction of 14-3-3 proteins with signaling molecules can localize, activate, inhibit, or stabilize the target molecules. Because 14-3-3 proteins are homo- and heterodimers, they have been considered as adaptor proteins that recruit and regulate the function of signaling molecules. They have been implicated in regulation of cell proliferation, cell cycle progression, apoptosis, and differentiation.