UGT8 Antikörper (AA 525-535)

Produktnummer ABIN1104921

Produktdetails anti-UGT8 Antikörper

Target: UGT8 (UDP Glycosyltransferase 8 (UGT8))

Bindungsspezifität: AA 525-535

Reaktivität: Human, Rind (Kuh), Hund, Meerschweinchen, Pferd, Kaninchen, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser UGT8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: Human UGT8. Reported variants represent identical protein: NP_001121646.1, NP_003351.2.

Homologie: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Dog, Bovine, Elephant, Panda, Horse, Rabbit, Porcine, Opossum, Guinea pig (100%) Zebra finch, Platypus, Lizard (91%) Rat, Turkey, Chicken, Xenopus, Zebrafish (82%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-NGKYKRNGHIK from an internal region of human UGT8 (NP_003351.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Dog, Bovine, Elephant, Panda, Horse, Rabbit, Porcine, Opossum, Guinea pig (100%), Zebra finch, Platypus, Lizard (91%), Rat, Turkey, Chicken, Xenopus, Zebrafish (82%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: Peptide-ELISA (1:64000), WB (1 - 3 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: In transfected MCF7 transiently expressing Human UGT8 a band of approx. 65kD is observed. This band is not observed in the non-transfected MCF7. The calculated molecular size is 61.5kD according to NP_003351.2. Recommended concentration, 1-3 μg/mL. Preliminary experiments gave an approx 50kD band in Human -Brain lysates and 48KDa in Rat Brain lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 61.5kD according to NP_003351.2. The observed band was successfully blocked by incubation with the immunizing peptide.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu UGT8

Target: UGT8 (UDP Glycosyltransferase 8 (UGT8))

Andere Bezeichnung: UGT8 (UGT8 Produkte)

Synonyme: Xlcgt antikoerper, cgt antikoerper, ugt4 antikoerper, AI850488 antikoerper, AW455908 antikoerper, Cgt antikoerper, Ugt8 antikoerper, mCerGT antikoerper, CGT antikoerper, UGT4 antikoerper, Ugt8a antikoerper, UDP glycosyltransferase 8 antikoerper, UDP galactosyltransferase 8A antikoerper, UGT8 antikoerper, ugt8 antikoerper, Ugt8a antikoerper, Ugt8 antikoerper

Hintergrund: Name/Gene ID: UGT8

Synonyms: UGT8, Cerebroside synthase, CGT, UGT4, UDP glycosyltransferase 8

Gen-ID: 7368

NCBI Accession: NP_003351

UniProt: Q16880

Pathways: Cell-Cell Junction Organization